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An Assist Trophy is an item that, when grabbed, summons a Non-Playable Character that does an effect to help the summoner, before disappearing. Choices of various origins to quickly aid the holder, not to mention some assistants get in the way more than they help. Once an Assist Trophy is grabbed, it is automatically activated (though this is not very convenient), assuming the holder is standing on the ground, and not in a jump, attack, or hitstun. Only one Assist Trophy (and the character it releases) can be in a match at once. The next Assist Trophy will not appear until the data for the character it will release is fully loaded. As shown in Lawl Nova, there is an option in which Assist Trophy characters can be turned on or off, except Dio.

Original

Character Universe Description
Best Benders The Last Airbender
Big Rig Big Rigs: Over the Road Racing
Chester A. Bum That Guy with the Glasses
Coconuts Sonic SonicSymbol
Denny Wiseau Films
Dr. Applecheeks Tom and Jerry
El Fua Nayarit en Línea
Ganon The Legend of Zelda ZeldaSymbol
Gnorris I.M. Meen
Kiko El Chavo del Ocho
Kyle Justin Angry Video Game Nerd
Mario Mario MarioSymbol
Mr. Six Six Flags
Persian Emissary 300
Poniko Yume Nikki
Wilford Brimley Liberty Medical

Lawl X

Character Universe Description
Abridged Tristan Yu-Gi-Oh!
Chiyo Azumanga Daioh
Demyx Kingdom Hearts
Freddie Freaker Freak Phone
Grunkle Stan Gravity Falls
Guy Who Moves His Head Aladdin (Dingo Pictures)
Jonny Sarhanis Ton of Cash
Jude 6teen
Kyary Pamyu Pamyu PonPonPon
Miyuki Lucky Star
Monika Doki Doki Literature Club
Oney Cloud Pointy Bits
Oney Marge Game Grumps Animated The Simpsons Hit & Run
Pein Naruto
Peter Griffin Family Guy
Proto Protogent
Ren Ren & Stimpy
Retro Ric Flair WWE
Riley Freeman The Boondocks
Ross Game Grumps
Stingy LazyTown
The Great and Powerful Trixie My Little Pony: Friendship is Magic
Thomas the Tank Engine Thomas the Tank Engine
Thresher Plumbers Don't Wear Ties

Lawl Nova

Character Universe Description
60's Electro Spider-Man
Anna Disney
Best Cosmo The John Show
Crocker The Fairly Odd Parents
Dio Brando JoJo's Bizarre Adventure
Eario Brawl of the Family
Egghead Batman
El Gordaco ???
Gameboy Captain N: The Game Master
Gamzee Makara Homestuck
Hans Moleman The Simpsons
Howard The Big Bang Theory
HowToBasic YouTube
I Like Trains Kid asdfmovie
John Travolta Actor
Kuzco Disney
Lemmings Lemmings
Marco Diaz Star vs. the Forces of Evil
Militron The Legend of Zelda
Norm Phineas and Ferb
Nosferatu Spongebob Squarepants
Soos Gravity Falls
Thin Cat & Fat Cat America's Funniest Home Videos
Tom Dickson Will It Blend
Toon Funky Kong Donkey Kong
Toon Roy Awkward Zombie
Toon Stan Monkey Island
Worst Dinosaurs Dingo Pictures

Lawl Liquid Crystal

Character Universe Description
Alphys Undertale
Annoying Toon Mr. Weebly
Best Patrick CRINGE YouTube
Boothworld Ind. Boothworld Industries
Coily Q*Bert
Destructive Robot MugiMikey
Malleo YouTube Poop
MS Paint Girl Sporum Dimensional Clash
Posessed Disney Stitch Teddy Disney/Hoaxes
Spingtrap Five Nights at Freddy's
Tank Sniper World of Tanks
The Gatekeeper NIGHTMARE: The Video Board Game